(May 13, 2011)
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Director: Kuniaki Ishii MD, Ph.D.
Associate Professor: Michililko SATO Ph.D.
The laboratory started as an Instrumentai Center in Yamagata University School of Medicine in 1981. Next year the laboratory moved to a new building between the building of Basic Medicin and Clinical Medicine. In April, 1983, the laboratory has changed the system. The name of the center, accordingly, changed to 'Central Laboratory for Research and Education'. There are 5 divisions, morphology, physiology, biochemistry, cell culture and the laboratory for common use.
The division provides facilities for ultrastructural analysis through its Electron Microscope Laboratory. The EM Laboratory houses two electron microscopes (Hitach H-7100 with CCD camera, S-5000S) which provide high-resolution transmission and scanning capabilities. Supporting apparatus such as ultramicrotomes, freeze-fracture equipment, as well as a fully equipped photographic laboratory are available.
A Carl Zeiss LSM-510 Meta confocal laser scanning microscope has been installed recently. A r/Kr mixed gas laser optics (458/477/488/514nm) and both 543 and 633 He/Ne laser optics with inverted microscope allow three channels to be monitored simultaneously at high resolution. Some very good data have already been generated.
A new fluorescence inverted microscope, Leica MD 3000B equipped with cooled CCD camera DP-70 (Olympus) has been available in the division.
This division provides facilities for the information processing architectures especially for the medical and biological sciences collaborated with I-ida branch of General information processing center of Yamagata University. The laboratory has several personal computers (Macintosh and Windows XP machines) provides DNA and Protein sequence analysis (Genetyx), image acquisition, image processing, optical character recognition, surface rendering, presentation graphics and animation services (full colour printers, Pictorography and Pictrostatt).
The division provides facilities for DNA analysis. Two DNA sequencers (ABI, 3130 (4 capillaries ) and 3500xl (24 capillaries) capillary DNA analyzer ) provide the good results of nucleotide sequence over 700-900 bases. In addition, an automated thermocycler (Veriti Lifetechnologies and TP600 Takara) is available to users for PCR.
4Peaks for Mac OS 10.3, FinchTV for Mac OS 10.3, Windows XP and UNIX, and Chromas for windows machine.
The Core is also equipped with automated technology to perform quantitative chores: Capture of isotope decay by imaging (Fuji film,BAS2000), Quantitative measurement of chemical and biological phosphorescence by luminometer (ATTO, AB2100 and Berthord, LB9507).
Capture of gel imaging by densitomitor (ATTO, Printograph) and Cooled CCD-camera system.
The division recently houses a real time PCR, ABI 7500 Fast, LightCycler System (Roche) , a powerful micro ultracentrifuge (Hitachi CD-150GXL). The ABI 7500 Fast and LightCycler system permits a real time PCR with a online monitoring of the results. A PCR with 30-40 cycles can be completed within 30 - 40 minutes.
There are several rooms for cell culture and the experiment for using bacteria (not for medical purpose). Several incubators and cabinets are available in the room. Bio-hazard room (P2) is also included in the division.
safty cabinet
laser micro-disection system (PALM)
There are several rooms for common research. The division houses Cell Analyzer (BD, FACSCalibur).
(1) FACSAria
(2) Sorting Divice
(3) Computer and printer
